Immunoaffinity chromatography as a means of purifying legumin from Pisum (pea) seeds
نویسندگان
چکیده
منابع مشابه
Nucleotide sequence of an A-type legumin gene from pea.
The A-subfamily of legumin genes encodes the predominant 11Sclass of seed storage proteins in pea (Pisum sativum L.). We have determined the complete nucleotide sequence of one such gene (Leg A2) located on a 4.2 kb EcoRl fragment and encoding a mature subunit with a deduced molecular weight of 56,929 Daltons. The coding region spans 1,825 bp (including three short introns) and exhibits a high ...
متن کاملRole of nitrogen content of pea (Pisum sativum L.) on pea aphid (Acyrthosiphon pisum Harris) establishment
The leaf nitrogen content is generally accepted as an indicator of food quality and as a factor affecting host selection by phytophagous insects. The alate pea aphids (Acyrthosiphon pisum Harris, Aphididae) were given a choice among non-nodulated pea plants (Pisum sativum L.) supplied with one of four nitrate-N levels (0, 3, 15 and 30 mM). When whole plants were exposed to aphids for 7 days, th...
متن کاملIsoelectric-focusing properties and carbohydrate content of pea (Pisum sativum) legumin.
Legumin from pea (Pisum sativum) is a molecule made up of six pairs of subunits, each pair consisting of an ;acidic' subunit (mol.wt. about 40000) and a ;basic' subunit (mol.wt. about 20000) linked by one or more disulphide bonds. The heterogeneity of legumin has been investigated by isoelectric focusing; undissociated legumin could not be focused satisfactorily, but legumin subunits could be a...
متن کاملHomology between legumin-like polypeptides from cereals and pea.
The presence of legumin-like constituents within the globulin fractions of wheat (Triticum aestivum), rye (Secale cereale) and corn (maize, Zea mays) was demonstrated. Two-dimensional analysis of wheat globulins in the presence and absence of a reducing agent revealed the existence of reducible approximately 60 kDa polypeptides. Western-blot analysis with 125I-labelled antibodies raised against...
متن کاملRespiratory potential and Se compounds in pea (Pisum sativum L.) plants grown from Se-enriched seeds.
Selenium (Se) has been proved to be an essential element for humans and animals. However, less is known about its effects on plants. Pea plants were treated foliarly once (OT) and twice (TT) with Se solution during their flowering period. Seeds obtained from these plants contained 383 and 743 ng Se g(-1), respectively, and, together with control seeds from untreated plants (UT) containing 21 ng...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1979
ISSN: 0264-6021
DOI: 10.1042/bj1770509